This reaction would contain only low concentrations of the dNTPs and relied upon brief incubation times, so that DNA synthesis would stall at random positions along the template and yield a population of DNA fragments with varying lengths. These unfinished DNA fragments were then purified and used as templates in four “minus” and four “plus” reactions.
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In fact, demand in the Chinese-style neighborhood snack segment is enormous. Shaxian Snacks and Hangzhou Xiaolongbao each have more than 10,000 and 30,000 stores nationwide, respectively. Although Yuanji Foods, with over 4,000 stores (nearly 1,500 in the Yangtze River Delta), ranks among the leaders, most brands in the sector are still mainly mom-and-pop operations, with weak quality control and fragmented supply chains. A comparable listed company in the same category, Babi Food, also focuses on neighborhood breakfast and snack scenarios and has achieved scaled expansion through franchising. However, Babi Food is positioned as more affordable, with an average ticket size of RMB 12–15—well below Yuanji Foods’ roughly RMB 20—while offering a broader product range that includes buns, steamed buns, and other breakfast items. Its supply-chain buildout is also more mature, giving it an edge in gross margin.